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. 2020 Feb 20;133(4):424–434. doi: 10.1097/CM9.0000000000000654

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Copyright © 2020 The Chinese Medical Association, produced by Wolters Kluwer, Inc. under the CC-BY-NC-ND license.

This is an open access article distributed under the terms of the Creative Commons Attribution-Non Commercial-No Derivatives License 4.0 (CCBY-NC-ND), where it is permissible to download and share the work provided it is properly cited. The work cannot be changed in any way or used commercially without permission from the journal. http://creativecommons.org/licenses/by-nc-nd/4.0

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Masson's trichrome staining and semi-quantitative analysis for the revealing of collagen and smooth muscle fiber in MTs. Top: Masson's trichrome staining compared the formation of collagen fiber and smooth muscle fiber between the non-induced MTs group and the induced MT group. Bottom: Semi-quantitative analysis exhibited a statistical significance in the difference between these two groups. ∗P < 0.05, comparison between the non-induced MTs and induced MTs. Scale bar = 20 μm. IOD: Integral optical density; MTs: Microtissues.