Fig 3. IFN-α induced ISG20 degrades m⁶A methylated HBV RNA via YTHDF2.

(A) HBV-WT, HBV-M1, HBV-M2 and HBV-M3 plasmids were separately co-transfected with FLAG-YTHDF2 into HepG2 cells and incubated for 48h until harvest and IFN-α was added (2000 IU/ml) 24h before harvesting the cells. After preparing the lysates FLAG-IP was done and probed with FLAG and ISG20 antibody after Western Blot. (B) RNA was isolated from the final eluted products from co-IP experiment and checked for pg/pc RNA expression by RT-qPCR. The data for this figure are from two independent experiments and the bars represent the mean ± SD.