Table 2. The oligos used to generate BZLF1 shRNA.
Name | Oligo |
---|---|
Forward Oligos | |
shRNABZLF1.1F | 5' CCGG - AACCCAGAATCAACAGACTAACC CTCGAG GGTTAGTCTGTTGATTCTGGGTT - TTTTTG 3' |
shRNABZLF1.2F | 5' CCGG - AACCTGGAGACAATTCTACTGTT CTCGAG AACAGTAGAATTGTCTCCAGGTT - TTTTTG 3' |
shRNABZLF1.3F | 5' CCGG - AATGCTTATCAAGCTTATGCAGC CTCGAG GCTGCATAAGCTTGATAAGCATT - TTTTTG 3' |
Reverse oligos | |
shRNABZLF1.1R | 5’ AATTCAAAAA - AACCCAGAATCAACAGACTAACC CTCGAG GGTTAGTCTGTTGATTCTGGGTT |
shRNABZLF1.2R | 5’ AATTCAAAAA - AACCTGGAGACAATTCTACTGTT CTCGAG AACAGTAGAATTGTCTCCAGGTT |
shRNABZLF1.3R | 5’ AATTCAAAAA - AATGCTTATCAAGCTTATGCAGC CTCGAG GCTGCATAAGCTTGATAAGCATT |