Figure 2.

Generation of SMC‐specific Jak2‐knockout mice. Schematic diagram of transgenic mice used to generate Jak2‐CKO and Jak2‐C mice (A). PCR analysis of tail genomic DNA to determine the presence of the floxed null allele (B). Western blot analysis to confirm Jak2 depletion in pulmonary arteries (C; n = 3 per group) and cardiac muscle (D; n = 3 per group). Immunohistochemistry analysis to confirm Jak2 depletion in PASMCs (E; n = 3 per group). Coimmunostaining results of p‐STAT3 and α‐SMA in lung sections from Jak2‐C and Jak2‐CKO mice (F). All images were taken at an original magnification of ×400. The data are represented as the mean ± SEM. ***P < .001