Figure 6.

JAK2/STAT3 promoted HPASMC proliferation programming by enhancing cyclin A2 expression following hypoxic exposure. Real‐time PCR to determine cyclin A2 (A), cyclin D1 (B), cyclin E1 (C), CDK2 (D) and CDK4 (E) expression in HPASMCs. Western blot analysis of cyclin A2 expression in HPASMCs (F). ChIP‐PCR results for analysis of p‐STAT3 binding activity to the CNNA2 promoter (G). Results for CNNA2 promoter luciferase reporter assays in HPASMCs (H). Coimmunostaining results of α‐SMA and cyclin A2 in lung sections from Jak2‐C and Jak2‐CKO mice after hypoxic (I; n = 10 per group) exposure for 28 days. All images were taken at an original magnification of ×400. The data are represented as the mean ± SEM. *P < .05; **P < .01; ***P < .001. CDK2, cyclin‐dependent kinase 2; CDK4, cyclin‐dependent kinase 4