Effects of ambient CO2 concentration (400 μmol mol−1) and elevated CO2 concentration (800 μmol mol−1) on the photosynthetic parameters of soybean plants at different growth stages. The values in the same column with different letters are significantly different (P < 0.05). Data are mean ± S.D. (n = 5 or 6); experiments were repeated three times. Pn, net photosynthesis; Gs, stomatal conductance; Tr, transpiration rate; WUE, water-use efficiency; Ci, intercellular CO2 concentration; Ca, atmospheric CO2 concentration; V1, first fully developed trifoliate leaves; R2, leaves from full flowering soybeans; R4, leaves from full pod stage of soybean.