Table 1.
Identification of the 24 significant lipids (top 18 are shown) in DMM plasma (n = 7 and 8 mice/group, for sham and DMM, respectively) using UHPLC-HR-MS global lipidomics platform and OPLS-DA analysis
| m/z | MS mode | Time (min) | VIP | Fold changea | Trendb | p valuec | Putative lipid biomarkerd | Confidence levele | Lipid class |
|---|---|---|---|---|---|---|---|---|---|
| 318.240 | POS | 0.32 | 11.0 | 1.27 | ↑ | 0.00749 | NAE 16:2 | 3 | Fatty acyl |
| 296.258 | POS | 0.28 | 10.0 | 1.49 | ↑ | 0.00731 | FA(18:3) | 3 | Fatty acid |
| 369.351 | POS | 2.86 | 8.0 | 1.39 | ↑ | 0.00598 | Cholesterol | 3 | Sterol lipid |
| 282.279 | POS | 0.73 | 7.9 | 1.39 | ↑ | 0.00893 | LCB 18:2;1 | 3 | Sphingolipid |
| 690.619 | POS | 2.81 | 7.2 | 1.37 | ↑ | 0.00656 | CE(20:4) | 2 | Sterol lipid |
| 666.618 | POS | 2.88 | 6.7 | 1.38 | ↑ | 0.00368 | CE(18:2) | 2 | Sterol lipid |
| 844.609 | NEG | 2.15 | 5.5 | 1.26 | ↑ | 0.0164 | PC(18:0/18:2) | 2 | PC |
| 613.492 | POS | 0.32 | 4.4 | 1.44 | ↑ | 0.00376 | CE(14:3) | 3 | Sterol lipid |
| 806.569 | POS | 1.45 | 4.4 | 1.17 | ↑ | 0.0129 | PC(38:6) | 3 | PC |
| 714.619 | POS | 2.77 | 4.2 | 1.43 | ↑ | 0.00841 | CE(22:6) | 2 | Sterol lipid |
| 804.551 | POS | 1.53 | 3.3 | 1.19 | ↑ | 0.0190 | PC(38:7) | 2 | PC |
| 473.401 | NEG | 1.37 | 2.8 | 1.07 | ↑ | 0.0130 | Sitosterol | 3 | Sterol lipid |
| 256.268 | POS | 0.6 | 2.7 | 1.24 | ↑ | 0.00797 | LCB 16:1;1 | 3 | Sphingolipid |
| 316.321 | POS | 0.79 | 2.6 | 1.13 | ↑ | 0.0217 | FA(19:0) | 3 | Fatty acid |
| 700.628 | POS | 2.72 | 2.3 | 1.43 | ↑ | 0.0114 | Cer 43:2;3 | 3 | Sphingolipid |
| 703.575 | POS | 1.53 | 2.2 | 1.39 | ↑ | 0.00680 | SM(d34:1) | 2 | Sphingolipid |
| 290.209 | POS | 0.27 | 1.8 | 1.31 | ↑ | 0.00866 | NAE 14:2 | 3 | Fatty acyl |
| 326.378 | POS | 0.66 | 1.8 | 1.45 | ↑ | 0.00334 | FA(22:1) | 3 | Fatty acid |
Lipids are sorted by their VIP scores (descending). Online databases METLIN, KEGG, HMDB and LIPIDMAPS were used to assign masses (m/z) to putative lipid species. Mass accuracy is considered less than 5 ppm. Fold change between DMM vs sham groups is shown. p values generated after applying FDR correction are given. Lipids identified by MS/MS experiments in Q Exactive with LipidSearch software) are highlighted in bold, when available. Lipid classes are also given
aDMM/sham group ratio (mean values from each group were used)
bDMM vs sham groups
cp value adjusted after FDR correction (5%) applied (GraphPrism v.6)
dLipid abbreviations: NAEN-acylethanolamine, FA fatty acid, LCB long-chain bases, CE cholesteryl ester, PC phosphocholine, Cer ceramide, SM sphingomyelin