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. 2020 Feb 3;225(2):705–734. doi: 10.1007/s00429-020-02029-2

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 6 — Retrogradely labelled hippocampo-subicular projecting trilaminar neurons in stratum oriens of the rat CA1 area. a Neurons in the CA1 area expressing EGFP following the injection of a recombinant adenovirus vector into subiculum (left, white arrowhead). Note some putative GABAergic neurons (insets, magenta arrowheads) retrogradely labelled together with the majority of CA1 pyramidal cells (contrast inverted epifluorescence images). b Hippocampo-subicular projecting EGFP-labelled cells in stratum oriens co-expressing M2 and decorated by mGluR8a+ terminals (epifluorescence micrograph). c Single-section reconstruction of soma with dendrites (red, section thickness 70 μm) and local axon collaterals (blue) of one EGFP-labelled GABAergic neuron co-expressing M2 and innervated by mGluR8a+ terminals (b left). d Postsynaptic target distribution of the EGFP-labelled cell reconstructed in c. eleft Axon collateral with boutons (white arrows) of the EGFP-labelled cell (black, HRP with nickel-DAB) reconstructed in c and PV+ dendrites (cyan arrowheads, brown, HRP with nickel-free DAB) in stratum oriens (transmitted light micrograph). Note the contact (magenta double arrow) between one EGFP-labelled bouton and one dendrite of a local PV+ interneuron (middle, reconstruction of soma and dendrites). Right: electron micrograph of the same EGFP-labelled bouton (black, HRP with nickel-DAB) making a type II synapse (magenta double arrow) with the PV+ interneuron dendrite (HRP with nickel-free DAB) also receiving a type I synapse (cyan arrowhead) of unknown origin. f Electron micrographs of EGFP-labelled boutons (black, visualised using HRP reaction and nickel-DAB as chromogen) of the cell reconstructed in c making type II synapses (magenta arrows) with a pyramidal cell dendrite (left, d) and an interneuron dendrite (right, d). Note the type I synapses (cyan arrowheads) received onto the spine (left, s) emanating from the dendrite and onto the shaft (right), respectively, identifying the postsynaptic targets as pyramidal cell and interneuron. CD, Sprague–Dawley; IN, interneuron; PC, pyramidal cell; unID, unidentified; + , immunopositive; −, immunonegative; slm, stratum lacunosum-moleculare; sr, stratum radiatum; sp, stratum pyramidale; so, stratum oriens; alv, alveus; sub, subiculum; scale bars 0.5 mm in a, 100 μm in insets of a and for reconstructions in c, e, 10 μm in b and for epifluorescence images in c, 0.5 μm in d and for electron micrograph in e, 25 μm for light micrograph in e