Fig. 13.

OGDH immunolabeling in the human cerebral cortex in relation to microglia and oligodendrocyte markers. a OGDH isoform 1/2 (green) and Iba1 (red) immunolabeling suggest a distinct distribution of OGDH-containing cells and microglia. In low magnification, OGDH- and IBA1-co-immunoreactivity may appear, but this notion is rejected in high magnification microphotographs. b A high magnification, optically 1 µm-thick confocal image of a cerebral cortical section double stained with OGDH isoform 1/2 (green) and Iba1 (red) demonstrates that larger, neuronal cells are single labeled for OGDH isoform 1/2. Several small cells are IBA1-positive. These cells are not labeled with OGDH isoform ½, suggesting that that microglial cells are negative for OGDH isoform 1/2. c A cerebral cortical section double labeled with OGDH isoform 1/2 (green) and the established oligodendrocyte marker myelin basic protein (MBP; red). The latter shows the typical distribution of myelinated fibers in the cerebral cortex. d A high magnification confocal image shows the lack of co-localization between OGDH isoform 1/2 and MBP-positive structures (red). Scale bars = 200 µm for a and c, 25 µm for b, and 50 µm for d