Fig. 15.

DLD immunolabeling in the human cerebral cortex in relation to neuronal and glial markers. a DLD-immunoreactive (DLD-ir) cells are present in layer 1 and also in deep layers of the cerebral cortex. The density of labeled cells is particularly high in layer 1 and the pyramidal layers. b DLD (green) and fluorescent Nissl staining (red) show that most cells are yellow, that is double-labeled in the cerebral cortex. c A cerebral cortical section double labeled with DLD (green) and the established astrocyte marker glial fibrillary acidic protein (GFAP; red) to show that most astrocyte contain DLD. b1–3 A high magnification confocal image of the cerebral cortical section double labeled with DLD (green) and fluorescent Nissl staining (red) demonstrates double labeling of the cells. The arrows point to some of the double-labeled cells. b1 shows the green channel, b2 the red channel, and b3 the merged figure. c1–3 A high magnification confocal image of the cerebral cortical section double labeled with DLD (green) and GFAP (red) demonstrates the presence of DLD in astrocytes (arrows). The arrowheads in turn point to neurons, which contain DLD, but do not express GFAP. c1 shows the green channel, c2 the red channel, and c3 the merged figure. Scale bars = 300 µm for a, 200 µm for b, 100 µm for c, and 20 µm for b1–3 and c1–3