Fig. 1. The Weimberg pathway for d-xylose conversion in C. crescentus.

The individual reactions of the pathway and the corresponding enzymes catalysing them (a), the d-xylose operon with the respective genes (xylBCDXA, C. crescentus NA1000) and encoded proteins (b), the change in standard Gibbs’ free energy for the oxidative Weimberg pathway¹⁴ (c), as well as the recombinant proteins after purification (SDS-PAGE and Coomassie staining) (d) are shown. For d-xylonolactone, the spontaneous, non-enzymatic conversion to d-xylonate is indicated (dotted line) (a). Abbreviations for enzymes and intermediates are given in the Fig. 1a; M, molecular weight marker. The source data underlying Fig. 1c are provided as a Source Data file.