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. 2020 Feb 27;10:3576. doi: 10.1038/s41598-020-60271-4

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© The Author(s) 2020, corrected publication 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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HDAC6 promotes the proliferation and tumorigenicity of human EC cells. (A) qRT-PCR showed HDAC6 mRNA levels in Ishikawa and AN3C cells after transfection with generated lentiviruses. (B) Western blotting showed HDAC6 protein levels in Ishikawa and AN3C cells after transfection with generated lentiviruses. (C) CCK-8 assay after overexpression and knockdown of HDAC6 in Ishikawa cells. (D) Colony formation assay after overexpression and knockdown of HDAC6 in Ishikawa cells. (E) Wound healing assay after overexpression and knockdown of HDAC6 in Ishikawa cells. Scale bar, 400 μm. (F) Transwell migration and invasion assays after overexpression and knockdown of HDAC6 in Ishikawa cells. Scale bar, 100 μm.*P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001.