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. 2020 Feb 27;10:3576. doi: 10.1038/s41598-020-60271-4

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© The Author(s) 2020, corrected publication 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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MiR-206 is downregulated in EC tissues and suppresses the proliferation, migration and invasion of endometrial cells in vitro. (A) qRT-PCR showed miR-206 levels in EC tissues (n = 46). (B) qRT-PCR showed miR-206 levels in the EC cell lines and the immortalized endometrial fibroblast cell line. (C) qRT-PCR showed miR-206 levels in Ishikawa and AN3C cells after transfection with generated lentiviruses. (D) CCK-8 assay of Ishikawa and AN3C cells transfected with the miR-206 inhibitor and miR-206 mimic. (E) Scratch wound assay of Ishikawa cells transfected with the miR-206 inhibitor and miR-206 mimic. Scale bar, 400 μm. (F) Transwell migration and invasion assays of Ishikawa cells after transfection with the miR-206 inhibitor and miR-206 mimic. Scale bar, 100 μm. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001.