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. 2020 Feb 27;11:1091. doi: 10.1038/s41467-020-14919-4

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© Crown 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — Cryopreserved PBMCs from n = 12 peanut-allergic (PA), n = 12 peanut-sensitized tolerant (PST), and n = 12 non-allergic (NA) healthy 1-year-old infants were thawed and underwent a 24 h stimulation with media (control), pure peanut protein (specific stimulation), or PMA/ionomycin (nonspecific stimulation). Samples were barcoded and stained with a panel of antibodies against 24 surface markers and 8 intracellular markers, and analyzed with a Helios Mass Cytometer. For data analysis, unsupervised computational analyses (clustering and visualization) and manual gating analyses were performed in parallel, along with statistical analyses to identify immune signatures significantly different between the clinical groups.