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. 2020 Feb 27;10:3629. doi: 10.1038/s41598-020-60594-2

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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STZ-induced type 1 diabetes mellitus reduces diastolic function. To determine diastolic performance, left ventricular anterior wall (a), left ventricular posterior wall (b), LV internal diameter (c), and end-diastolic volume (d) were assessed using a Vevo 3100. In addition, diastolic wall strain (e) was calculated using posterior wall thicknesses during systole and diastole. Bar graphs represent the mean ± SEM. Data were analysed with One-way ANOVA or Kruskal-Wallis test (*p < 0.05; **p < 0.01, ***p < 0.001, ***p < 0.0001 versus corresponding control; ^†p < 0.05, ^††p < 0.01, ^†††p < 0.001, ^††††p < 0.0001 versus the 6w STZ; ^‡p < 0.05, ^‡‡p < 0.01, ^‡‡‡p < 0.001, ^‡‡‡‡p < 0.0001 versus the 9w STZ; n = 12/controls and n = 14/STZ).