Table 2.
Primers used.
| Primer name | Primer Sequence 5′ to 3′ | Position on modified AIF1L allele |
|---|---|---|
| For genotyping | ||
| Aif1l_39300_F | GCTCCTGCACTCTGCATCTC | 14699–14718 (From WTSI*) |
| Aif1l_39300_R | ACCTTCCCCACTCTGTGCTC | 22236–22255 (From WTSI) |
| CAS_R1_Term | TCGTGGTATCGTTATGCGCC | 15053–15072 (From WTSI) |
| LacZ_2_small_F | ATCACGACGCGCTGTATC | 18203–18220 |
| LacZ_2_small_R | ACATCGGGCAAATAATATCG | 18291–18310 |
| Exon3 forward | GATATCTCTATAGTCGCAGTAGGC | 22147–22161 |
| Exon3 reverse | CTTTGAAGGCTGCGAGCTTTTCC | 22621–22643 |
| For cDNA analysis | Primer Sequence 5′ to 3′ | |
| Exon1_F | ATGTCGGTCGCACTCAGCAA | |
| Exon5_R | CTGACACCCCCTGTCACCTC | |
| Exon2_R | CCGATTGATCTCCTCGAGCCTC | |
| Adiponectin_F | GGTCTTCTTGGTCCTAAGGGTGAG | |
| Adiponectin_R | GCGGCTTCTCCAGGCTCTC | |
| Resistin_F | GCTGCTCCTGTGGCTCTGC | |
| Resistin_R | GGCTGCTGTCCAGTCTATCCTTG | |
| Leptin_F | AGGGAGGAAAATGTGCTGGAGAC | |
| Leptin_R | GATACCGACTGCGTGTGTGAAATG | |
*WTSI- Wellcome trust Sanger Institute.