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. 2020 Feb 27;10:3566. doi: 10.1038/s41598-020-60600-7

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fluorescence micrographs of thoracic midguts with cardia section and stomodeal valve of Lutzomyia spp. females on days 8 postinfection. Cardia of Lu. longipalpis (A–C) and Lu. migonei (D–F) coinfected by Leishmania (Viannia) braziliensis (green) and Leishmania (Leishmania) infantum (red). Control uninfected gut of Lu. longipalpis (G–I). (A,D,G) images from red fluorescence, (B,E,H) images from green fluorescence and (C,F,I) merged images, scale bar: 5 mm.