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. 2020 Feb 27;10:3653. doi: 10.1038/s41598-020-60443-2

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Effect of PG-CAT treatment on RSV disease in mice. BALB/c mice were either un-infected and treated with PBS or PG-CAT (PBS – PBS or PG-CAT – PBS), or infected and D0 and treated at D(−1) and D(1) with PBS, PG only, or PG-CAT (PBS – RSV, PG – RSV, and PG-CAT – RSV) (A). Mice were assessed for: body weight loss (B); clinical disease score (C); lung viral titers by plaque assays at day 5 p.i. (D); BALF total protein concentration at day 2 p.i. (E); airway obstruction by whole body plethysmography (F). Data are mean ± SEM. Significance was determined using repeated measures ANOVA (B, C, and F), one-way ANOVA (E) and student’s t-test (D). Tukey’s multiple comparison analysis was used for inter-group analysis. *p < 0.05, **p < 0.01, and ***p < 0.001 is significance for PBS – RSV vs PG-CAT – RSV. ^p < 0.05, ^^p < 0.01, and ^^^p < 0.001 is significance for PG – RSV v PG-CAT – RSV.