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. 2020 Feb 27;11:1101. doi: 10.1038/s41467-020-14931-8

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — a Immunofluorescent staining of the cerebellum from AAV-TBP-injected wild-type mouse brains. Antibodies to TBP, NeuN, and calbindin were used. Scale bar = 100 μm. b Immunofluorescent staining of the striatum from AAV-TBP-injected wild-type mouse brain. Staining with antibodies to TBP and DARPP32, a marker for medium spiny neurons, showed a reduced expression of DARPP32 in a polyQ length-dependent manner. Scale bar = 20 μm. c Immunofluorescent staining of the prefrontal cortex from AAV-TBP-injected wild-type mouse brain with anti-TBP and anti-NeuN antibodies. Scale bar = 20 μm. d Quantification of neuronal loss in the cerebellum, striatum, and prefrontal cortex from AAV-TBP-injected wild-type mouse brains. One-way ANOVA followed with Tukey’s multiple comparisons test was performed; cerebellum calbindin, F = 166.7; cerebellum NeuN, F = 31.65; striatum, F = 638.1; prefrontal cortex, F = 8.504, **P < 0.005, ***P < 0.0005, ****P < 0.0001, n = 4 mice per group, three images per brain region from each mouse were used to count. Data are represented as mean ± SEM. Source data are provided as a Source Data file.