Fig. 4. Drive allele inheritance.

Females and males heterozygous for the drive allele and the Cas9 allele were crossed with w¹¹¹⁸ individuals, and their progeny were phenotyped for dsRed, which indicates the presence of the drive. Females showed biased inheritance of the drive, since many individuals without the drive had two disrupted copies of h and thus were nonviable. Half of the progeny of male drive heterozygotes received the drive, since individuals that received a disrupted copy of h still received a functional wild-type copy from their mother that remained undisrupted in the absence of maternal Cas9 activity. The size of the dots represents the sample size of adult progeny from a single drive individual. The rate estimates and corresponding estimates of the standard error of the mean (SEM) are displayed, obtained from pooling offspring from all crosses of the same type together (n = 1598 for the progeny of females and n = 706 for the progeny of males). An alternative statistical analysis that accounts for potential batch effects was also performed but yielded overall similar rates with only slightly increased error estimates (Supplementary Methods, Source Data: Data Sets 1−2).