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. 2020 Feb 27;10:3590. doi: 10.1038/s41598-020-60341-7

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Magnification of the buccal side in the immediate model (a–d) and the conventional model (e–h). The amount of newly formed bone above the defect in the buccal side was similar regardless of the peri-implantitis model. However, the new bone on the buccal side was greater in the conventional model than in the immediate model. Multiple stains on undecalcified ground sections (a,c), 2X original magnification (a–d); bone labelling at 4 weeks (green; oxytetracycline HCl) and 8 weeks (orange; xylenol orange). Yellow line, bottom of the defect; white line, dental implant; pink line, outline of newly formed bone. B; buccal, L; lingual, BG; bone graft, NB; new bone, OB; old bone; bars indicate the bottom of the defect.