Fig. 3. Breakage of the casted hydrogel is suppressed in SDC-based tissue clearing.
a, b Maximum projection of volumetric images of the neocortex. The vascular-casted samples were delipidated using SDS a or SDC b and were immersed in ScaleCUBIC-2. Some penetrating vessels were severed (arrowheads) in the SDS-treated group, whereas such breakages were rarely observed in the SDC-treated group. Scale bar = 100 μm. c The percentages of the broken penetrating vessels in the brain treated with SDS and SDC (n = 6 and 9 brains, respectively; box plots indicate median and 25−75% interquartile ranges, ***P = 8.84 × 10−7, t13 = 8.70, Student’s t-test). Source data are provided as a Source Data file.