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. 2020 Feb 27;10:3579. doi: 10.1038/s41598-020-60618-x

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Functional RNA quality assessment by fragment size PCR Endpoint assay and qRT-PCR technique. Six amplicons of different length (73, 156, 257, 347, 400 and 436 bp) of the HPRT1 gene were amplified using cDNA purified from RNL, SF, SF-OCT and FFPE lung tissue samples (n = 20). (a) Images of electrophoresis gels showing the PCR products for the six amplicons tested for patient 17 in all preservation methods compared: RNL (top of the left), SF (top of the right), SF-OCT (bottom of the left) and FFPE (bottom of the right). Full-length gels are presented in Fig. S1. (b) Cq mean values and standard deviation (SD) obtained by each amplicon in each preservation methods tested (n = 20). SDs are represented as error bars. For RNL, SF and SF-OCT, SD was too small to be consistently represented in the graph.