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. 2020 Feb 27;10:3579. doi: 10.1038/s41598-020-60618-x

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Expression levels of housekeeping genes when evaluating the impact of thawing time before RNA extraction. RNL (a,b), SF (c,d) and SF-OCT (e,f) tissue samples were submitted to increasing thawing times (0, 1, 3, 6, 12, 30 and 45 minutes) at room temperature and Cq values of HPRT1 (purple), SNRPD3 (green) and Jun (yellow) genes were measured. Time points were compared using two-way ANOVA with multiple comparisons. All time points highlighted with *showed a significant difference compared to the control condition (0 minutes) with a p < 0.01.