Figure 5.

Human skin-derived organoids delivered by the AMAD improved wound healing. (A) Representative images of the mouse wound splinting model after spraying of the skin organoids; the medium without cells was used as the control group. (B) Analysis of wound size (%) of each group, and the wound area over time were measured as a percent of the original area, n = 3. (C) Wound sections on 7, 14, and 21 days after spraying were stained with H&E for general observation of skin layers. (D) Immunohistochemical staining of Ki67, CK14, and CK10 on the skin wound treated by spraying of epidermal organoids vs. control (saline).