| Subject Area: | Biochemistry, Genetics and Molecular Biology |
| More specific subject area: | Metabolic profiling |
| Protocol name: | IPS-CM functional characterization |
| Reagents/tools: | Included in each section of the protocol |
| Experimental design: | Functional and beating induced pluripotent stem cells derived cardiomyocytes are cultured in a standard conditions or in the presence of 13C labeled glucose. Cells are harvested through the developed protocol, followed by mass spectrometry assays to analyze small metabolites, glucose uptake and lactate/pyruvate production. |
| Trial registration: | N/A |
| Ethics: | N/A |
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