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. 2019 Dec 17;44(1):230–238. doi: 10.1007/s12639-019-01186-4

Table 2.

Conjugation of standard HRP to AC IgG using different concentrations of glutaraldehyde

Sample numbera Protein (mg) Activity (units) Specific activity (units/mg protein) Recovery (%) ELISA titer
1 2.5 1000 400 100
2 3.2 750 234 75 1: 10
3 3.2 850 265 85 1: 15
4 3 800 266 80 1: 50

aSample number: 1, Standard HRP; 2, HRP-AC IgG conjugate (using 1% glutaraldehyde), 3, HRP-AC IgG conjugate (using 5% glutaraldehyde) and 4, HRP-AC IgG conjugate (using 10% glutaraldehyde)

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