Table 2.
Sample numbera | Protein (mg) | Activity (units) | Specific activity (units/mg protein) | Recovery (%) | ELISA titer |
---|---|---|---|---|---|
1 | 2.5 | 1000 | 400 | 100 | – |
2 | 3.2 | 750 | 234 | 75 | 1: 10 |
3 | 3.2 | 850 | 265 | 85 | 1: 15 |
4 | 3 | 800 | 266 | 80 | 1: 50 |
aSample number: 1, Standard HRP; 2, HRP-AC IgG conjugate (using 1% glutaraldehyde), 3, HRP-AC IgG conjugate (using 5% glutaraldehyde) and 4, HRP-AC IgG conjugate (using 10% glutaraldehyde)