FIG. 3.

Impaired growth occurred at larva to juvenile transitory phase of homozygous thrab 1-bp insertion (m/m) mutant fish. (A) Growth characteristics and gene expression of thrab 1-bp ins mutant fish for juveniles (28 dpf) and (B) larvae (15 dpf). Body length (a), body width (b), and body weight (c) were measured for juveniles (A-I) (number of fish = 22–29) and for larvae (B-I) (number of fish = 23–71). Relative mRNA expression of gh1, smtla, and smtlb was determined through RT-qPCR using the primers listed in Supplementary Table S1 for juveniles in the pituitary (A-II) and for larvae in head (B-II). The genotypes are indicated. The data are expressed as mean ± SE (n = 3; the p-values are indicated). Two-tail unpaired t-test, p-adjusted <0.05. dpf, days postfertilization; RT-qPCR, real-time quantitative PCR.