Table 1.
Comparison of the C-Terminal Amino Acid Sequences of Wild-Type and Mutant Thyroid Hormone Receptor α Generated Through CRISPR/CAS9-Mediated Targeted Mutagenesis
| Protein | C-terminal amino acid sequence |
|---|---|
| The thraa gene | |
| Wild-type TRα1L | -FPPLFLEVFEDQEGSTGVAAQEDGSCLR*-428 |
| 4-bp deletion ThraaPhe404Leufs*22 |
-FPPLLRSSRIRREALEWQHRKTVPA*-425 |
| 8-bp insertion ThraaLeu405Glufs*6 |
-FPPLFEDQEV*-410 |
| Wild-type TRα1S | -FPPLFLEVFEDQEV*-414 |
| 4-bp deletion ThraaPhe404Leufs*10 |
-FPPLLRSSRIRRC*-412 |
| 8-bp insertion ThraaLeu405Glufs*6 |
-FPPLFEDQEV*-410 |
| The thrab gene | |
| Wild-type TRαB | -HASRFLHMKVECPTELFPPLFLEVFEDQDV*-409 |
| 4-bp deletion ThrabThr393Profs*31 |
-HASRFLHMKVECPPSFPHFSWRSSRIRTCDVPANCGKRIRNVS*-422 |
| 1-bp insertion ThrabGlu394* |
-HASRFLHMKVECPT*-394 |
bp, base pair.