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. 2020 Feb 7;9(4):145–160. doi: 10.1089/wound.2019.0956

Figure 3.

Figure 3.

hsa_circ_0084443 inhibits keratinocyte migration. Scratch wound assay of keratinocytes transfected with 20nM si-hsa_circ_0084443 and si-control (A, B) or 0.1 μg hsa_circ_0084443 plasmid and empty vector (C, D) for 24 h (n = 4). Photographs were taken at the indicated timepoints after the scratch (A, C). The healing rates were quantified (B, D). Live-cell imaging using the IncuCyte® system of migrating keratinocytes with hsa_circ_0084443 knockdown (n = 12). Representative photographs of migrating keratinocytes at the indicated timepoints. Scale bar = 300 μm (E). The migration rates were quantified by measuring the area of the scratched region (F). Representative photographs of the transwell migration assay for keratinocytes with hsa_circ_0084443 knockdown (n = 3) (G) or hsa_circ_0084443 overexpression (n = 3) (I). The number of cells passing through the membrane was counted (H, J). *p < 0.05, **p < 0.01, and ****p < 0.0001 by unpaired two-tailed Student's t test (B, D, H, J) and by two-way ANOVA (F). Data are presented as mean + SD. Color images are available online.