Figure 1.

Intramuscular AAV transduction efficiency in control C57BL/6 mice. rAAV serotypes 1–10 were injected into the TA (3.5 × 10¹⁰ vg/TA). Mice were sacrificed 4 weeks postinjection and TA muscles were analyzed (n = 4–10 TA). (A) Cryostat sections of TA muscles of C57BL/6 mice injected with rAAV serotypes 1–10 carrying the nls-LacZ gene reporter and stained for β-galactosidase activity and laminin. Scale bar = 250 μm. (B) Quantification of cross-sectional β-galactosidase-positive nuclei by mm² (n = 4–10 TA). (C) Transduction efficiency is expressed as the β-gal-positive nuclei/mm² relative to the AAV genome copy number quantified by absolute TaqMan qPCR. *p < 0.05, **p < 0.01, ***p < 0.001, and ****p < 0.0001 ANOVA Tukey test. AAV, adeno-associated virus; ANOVA, analysis of variance; qPCR, quantitative PCR; rAAV, recombinant AAV; TA, tibialis anterior; vg, vector genome.