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. 2020 Jan 17;16:238–249. doi: 10.1016/j.omto.2020.01.001

Figure 3.

Figure 3

GSK2606414 Inhibits Reovirus-Induced GRP78 while Increasing PDI and Overall ER Resident KDEL Levels

FaDu and HN5 HNSCC 3D tumor spheroids were treated with GSK2606414 and reovirus for 96 h before spheroids were formalin fixed, paraffin embedded, and sectioned. Sections were stained by IHF for (A) GRP78, (B) KDEL, and (C) PDI. The ×20 field of view shown corresponds to 415 × 415 μm. Confocal images were quantified for areas of high expression and restricted to the peripheral 25 μM as previously outlined in Figure 2. Quantification for both FaDu and HN5 cells shown for (D) GRP78, (E) KDEL, and (F) PDI. Each point represents an independent experiment where each experiment is an average of four to eight spheroids. Data are ±SEM with statistical analysis by unpaired t test, *p < 0.05.