Fig. 2.
Knockdown efficiency in larval brains. (A) SLC25A46a protein levels in larval brains analyzed by Western immunoblotting. Proteins were extracted from larval CNS carrying elav > UAS-w-IR (w; UAS-w-IR/+; elav-GAL4/+) (a), elav > UAS-dSLC25A46a-IR17-183 (w; UAS-dSLC25A46a-IR17-183/+; elav-GAL4/+) (b), and elav > UAS-dSLC25A46a -IR217-383 (w; UAS- dSLC25A46a-IR217-383 /+; elav-GAL4/+) (c). The blotted membrane was probed with a rabbit anti-dSLC25A46a antibody. The band with an apparent molecular mass of 50 kDa corresponding to the dSLC25A46a protein is marked. The positions of size markers are also given on the left. (B) Quantification of the 50-kDa band. α-Tubulin was used as a loading control. The relative intensity of the 50-kDa band normalized to that of α-tubulin is shown as each bar. ****p < 0.0001, biological replication, n=4.