Fig. 4.
Knockdown of dSLC25A46a in pan-neurons causes an abnormal synapse morphology at NMJs in muscle 4 of third instar larvae. (A) Confocal images showing NMJs stained with anti-HRP IgG (a, d and g) and anti-Dlg IgG (red) (b, e and h). Merged images are shown in panels c, f, and i. The NMJs of flies carrying elav > UAS-w-IR (w; UAS-w-IR/+; elav-GAL4/+) (a-c), elav > UAS-dSLC25A46a-IR17-183 (w; UAS-dSLC25A46a-IR17-183/+; elav-GAL4/+) (d-f), and elav > UAS-dSLC25A46a-IR217-383 (w; UAS-dSLC25A46a-IR217-383 /+ ; elav-GAL4/+) (g-i) are shown. The proximal region and distal region of the synapse branch are shown by arrows and arrowheads, respectively. Examples of boutons are marked in panels c, f, and i. Scale bars indicate 20 μm. (B, C, D) Quantified data. In dSLC25A46a knockdown larvae, the longest branch length (B) and total length of synaptic branches (C) were shorter and bouton numbers (D) were smaller than control larvae. *p < 0.05 and **p < 0.01. n = 12.