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. 2020 Feb 19;8:65–75. doi: 10.1016/j.ibror.2020.02.001

Fig. 6.

Fig. 6

Knockdown of dSLC25A46a causes abnormal mitochondrial dynamics at NMJs in muscle 4 of third instar larvae. (A) Images show NMJs that were double-stained with mito-GFP (green) and anti-Dlg IgG (red). NMJs of flies carrying w; UAS-w-IR/+; elav-GAL4, UAS-mito-GFP/+ (a–d), w; UAS-dSLC25A46a-IR17-183/+; elav-GAL4, UAS-mito-GFP/+ (e–h) and w; UAS-dSLC25A46a-IR217-383 /+; elav-GAL4, UAS-mitoGFP/+ (i–l) are shown. Enlarged images of the region enclosed in panels c, g, and k are shown in panels d, h, and l, respectively. Examples of mitochondria are marked in panels a, e, and i. Scale bars indicate 10 μm. (B-D) Quantified data. In dSLC25A46a knockdown larvae carrying elav>mito-GFP,dSLC25A46a-IR17-183 (w; UAS-dSLC25A46a-IR17-183/+; elav-GAL4, UAS-mito-GFP/+, n = 12) and elav>mito-GFP,dSLC25A46a-IR217-383 (w; UAS-dSLC25A46a-IR217-383 /+; elav-GAL4, UAS-mito-GFP/+, n = 12), the area and size of mitochondria significantly differed from (B, C), whereas the number of mitochondria were similar to (D) in control larvae carrying elav>mito-GFP,w-IR (w; UAS-w-IR/+; elav-GAL4, UAS-mito-GFP/+, n = 12). *p < 0.05 and **p < 0.01. ns: p > 0.63 for larvae carrying elav>mito-GFP,dSLC25A46a-IR17-183 (w; UAS-dSLC25A46a-IR17-183/+; elav-GAL4, UAS-mito-GFP/+), and ns: p > 0.83 for larvae carrying elav>mito-GFP,dSLC25A46a-IR217-383 (w; UAS-dSLC25A46a-IR217-383 /+; elav-GAL4, UAS-mito-GFP/+).