Figure 2.

Improved efficacy in St/CDA vaccinated mice upon a lethal challenge with T. cruzi RA strain. Female C3H mice were vaccinated and 15–30 days after last dose were intraperitoneally infected with 1,000 blood trypomastigotes of T. cruzi RA strain (A) Parasitemia. (B) Area under the curve (AUC) of parasitemia up to 14 dpi. RU, relative units **p < 0.01, *p < 0.05 differences were calculated with respect to Se/PBS control group, one-way ANOVA + Bonferroni post-hoc test. (C) Weight loss. Results are expressed as percentage with respect to pre-infection **p < 0.01, *p < 0.05 at 17 dpi between the indicated groups, one-way ANOVA + Dunnett post-test. (D) Survival rate. Asterisks indicate significant difference with respect to Se/PBS control group. **p < 0.01, *p < 0.05 Log-rank test. n = 5–7 mice per group. Results are representative of three independent experiments.