Figure 10.

Comparison of anti-inflammatory effects of XBJ and its main components. Mouse primary peritoneal macrophages were seeded at 3.5 × 10⁵ cells/well in 24-well plates and cultured overnight. Cell were treated with XBJ (10 µl/ml), PAE (26.7 µg/ml), and HSYA (13.7 µg/ml) with or without Pam3CSK4 (100 ng/ml) for 3, 6, or 18 hours. The concentrations of IL-6 (A), TNF-α (B), and IL-12p70 (C) in the cell culture supernatant were detected by ELISA. Data were shown as mean ± SD (n=3). *^,**^,***Significantly different at p < 0.05, p < 0.01, and p < 0.001, respectively.