Figure 6.

XBJ inhibited the production of Pam3CSK4-induced inflammatory mediators in Raw264.7. Raw264.7 cells were seeded (2 × 10⁵ cells/well) in 24-well plates overnight and stimulated by Pam3CSK4 (100 ng/ml) with different concentrations of XBJ for 6 or 18 hours. Cell culture supernatants were collected and the concentrations of IL-6 (A), TNF-α (B), and IL-10 (D) were measured by ELISA. IL-1β (C) mRNA expression was examined by qRT-PCR. NO concentrations (E) was measured by Griess assay at 12 or 24 hours after stimulation. Data were shown as mean ± SD (n=3). *^,**^,***Significantly different at p < 0.05, p < 0.01, and p < 0.001, respectively.