Fig. 2.

Expression of OGT and OGA in intestine epithelial cells of CD is marginally altered. (a - f) Assessment of OGT and OGA in intestine tissues from healthy (n = 45), active CD (n = 36), and inactive CD (n = 22) individuals by IHC. Representative staining of OGT (a) and OGA (d) for ileal tissues from each group is presented. Scale bar = 50 µm. Percentage of samples with high or low expression of OGT (b) or OGA (e) in intestinal tissues are shown. H-scores of OGT (c) and OGA (f) for the staining are also displayed. (g) HCT116 cells were treated with AIEC strain LF82 as described in Fig. 1d. Whole cell extracts from the cells were used for the detection of OGT and OGA by western blot. GAPDH was detected for loading control.