Fig. 6.

Inhibition of O-GlcNAc reduces cell-associated AIEC LF82 accumulation. HCT116 cells in a 6-well plate were treated with 10 µM DON (a), knockdown of shR-OGT (b), or overexpression of OGA (c) for the suppression of the O-GlcNAc. Cells were exposed to 10 µM TG (d), knockdown of shR-OGA (e), or overexpression of OGT (f) for the upregulation of the O-GlcNAc. AIEC strain LF82 was used to infect the cells (MOI = 10) for the indicated time. After the infection, cells were washed and lysed for bacterial colonel formation assay. Total colony numbers per well were presented. Values represent the mean ± S.D. * P < .05 and ** P < .01 as compared with non-O-GlcNAc-manipulated groups, n = 3 (Student's t-test).