Table 2.
Association of KRAS gene mutation and RASSF1A aberrant promoter region methylation with the clinicopathological characteristics of NSCLC patients.
| S.no | Variables | No. of Patients |
KRAS Mutation (Exon 2) |
OR (95%CI) | p value |
RASSF1A methylation status |
OR (95%CI) | p value | |||
|---|---|---|---|---|---|---|---|---|---|---|---|
| Positive n (%) | Negative n (%) | No. of methylated samples n (%) | No. of Unmethylated samples n (%) | ||||||||
| 1. | Age at Diagnosis | ||||||||||
| ≤45Y | 13 | 3 (23.08) | 10 (76.92) | 0.632 (0.120–3.329) | 0.58 | 3 (23.08) | 10 (76.92) | 0.824 (0.261–2.589) | 0.13 | ||
| >45Y | 87 | 28 (32.18) | 59 (67.82) | 1 | 38 (43.67) | 49 (56.33) | 1 | ||||
| 2. | Gender | ||||||||||
| Male | 68 | 26 (38.23) | 42 (61.77) | 0.970 (0.327–2.873) | 0.95 | 30 (44.11) | 38 (55.89) | 0.540 (0.130–2.145) | 0.60 | ||
| Female | 32 | 5 (15.62) | 27 (84.38) | 1 | 11 (34.37) | 21 (65.63) | 1 | ||||
| 3. | Histological Type | ||||||||||
| Adenocarcinoma | 72 | 21 (29.16) | 51 (70.84) | 0.811 (0.257–2.588) | 0.01∗ | 34 (47.22) | 38 (52.78) | 0.621 (0.157–2.183) | 0.03∗ | ||
| Squamous Cell Carcinoma |
28 | 10 (35.71) | 18 (64.29) | 1 | 7 (25) | 21 (75) | 1 | ||||
| 4. | Smoking Status | ||||||||||
| Smokers | 62 | 25 (40.32) | 37 (59.68) | 4.899 (1.273–18.77) | 0.01∗ | 32 (51.61) | 30 (48.39) | 3.099 (1.167–4.443) | 0.01∗ | ||
| Non smokers | 38 | 6 (15.78) | 32 (84.22) | 1 | 9 (23.68) | 29 (76.32) | 1 | ||||
| Current smokers | 20 | 6 (30) | 14 (70) | 0.182 (0.047–0.697) | 0.01∗ | 12 (60) | 8 (40) | 0.282 (0.036–0.856) | 0.03∗ | ||
| Ex-smokers | 42 | 19 (45.23) | 23 (54.77) | 1 | 20 (47.61) | 22 (52.39) | 1 | ||||
| 5. | TNM Staging | ||||||||||
| Early stage (I and II) | 53 | 11 (20.75) | 42 (79.25) | 0.353 (0.112–1.116) | 0.05∗ | 11 (20.75) | 42 (79.25) | 0.359 (0.114–1.129) | 0.05∗ | ||
| Advanced Stage (III and IV) | 47 | 20 (42.55) | 27 (57.45) | 1 | 30 (63.82) | 17 (36.18) | 1 | ||||
| 6. | Distant Metastasis | ||||||||||
| Positive | 18 | 06 (33.33) | 12 (66.67) | 0.941 (0.319–2.775) | 0.03∗ | 10 (55.55) | 08 (44.45) | 0.821 (0.289–2.786) | 0.02∗ | ||
| Negative | 82 | 25 (30.48) | 57 (69.52) | 1 | 31 (37.80) | 51 (62.20) | 1 | ||||
| 7. | Smoking Level | ||||||||||
| Mild (≤10) | 5 | 03 (60.00) | 02 (40.00) | 1 | 0.64 | 0 (0.00) | 05 (100) | 1 | 0.72 | ||
| Moderate (≤40) | 30 | 12 (40) | 18 (60) | 0.550 (0.043–7.034) | 16 (53.33) | 14 (46.67) | 0.460 (0.023–7.045) | ||||
| Heavy (>40) | 27 | 10 (37.03) | 17 (62.97) | 0.308 (0.024–3.968) | 0.34 | 18 (67.66) | 9 (32.34) | 0.209 (0.014–3.799) | 0.22 | ||
| No-smoking | 38 | 6 (15.78) | 32 (84.22) | 1 | 7 (18.42) | 31 (81.58) | 1 | ||||
∗ significant (p < 0.05).