Figure 3.

Higher frequency of non-classical monocytes in patients with Hashimoto's thyroiditis. (A) HSNE-embedding depicting the local probability density of CD14⁺ Mo (upper first left) and subsets defined by Gaussian mean-shift clustering (lower first left). Relative expression of selected markers in embedded CD14⁺ Mo. (B) Heatmap showing ArcSinh5-transformed median expression of relevant markers within the clusters identified in (A). The dendrogram depicts hierarchical clustering of subsets. (C) Frequency of non-classical Mo (#6) in healthy controls (HC, n = 15) and patients with new-onset (N-T1D, n = 7) and long-standing (L-T1D, n = 9) type 1 diabetes, Hashimoto's thyroiditis (HT, n = 8), Graves' disease (GD, n = 7) and autoimmune Addison's disease (AD, n = 8). Dots represent individual samples and bars indicate median. One-way ANOVA with Tukey's test for multiple comparisons, *p < 0.05, **p < 0.01, and ***p < 0.001. HSNE, hierarchical stochastic neighbor embedding; Mo, monocytes.