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. 2020 Jan 23;6(2):213–225. doi: 10.1021/acscentsci.9b01125

Figure 1.

Figure 1

Herbal extracts showing Gq-mediated activity at 5-HT2C were selected for affinity MS screening. (A) Calcium mobilization elicited by three 5-HT2 subtypes treated with different herbal extracts or 5-HT. Full names of the herbs used are listed in the Methods section. NA, not active. (B–D) Dose–response characteristics of calcium mobilization elicited by top 5 extracts or 5-HT at 5-HT2C, 5-HT2B, and 5-HT2A. Crude extract concentrations were calculated from weight, assuming an average molecular weight of 500 Da for small molecule constituents. (E) Workflow of affinity MS-based 5-HT2C ligand screening. A putative ligand (green) and a nonspecific binder (blue) are distinguished based on the MS intensity of each compound detected in the 5-HT2C target vs control.