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. 2020 Feb 21;10:181. doi: 10.3389/fonc.2020.00181

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Copyright © 2020 Zhao, Zheng, Wang, Hou, Yang, Cheng, Che, Xie, Wang, Zhang, Kang, Liu, Pan, Qu, Hu and Fan.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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miR-1323 can promotes the migration of A549 cells through inhibiting Cbl-b. (A) A schematic view of miR-1323 predicted target genes through four miRNA databases of different mechanisms. The A549 cells were transfected with miR-1323 mimic or NC, (B) WB detected the expression of proteins and (C) Elisa detected the secretory level of IL-6 in A549 cells. (D) The bindng site of miR-1323 to Cbl-b 3′UTR. (E) The A549 cells were co-transfected with pMirTarget-Cbl-b WT (or MUT) plasmid, miR-1323 mimic (or NC), and pRL-TK. The dual luciferase reporter assay detected the activity of luciferase. The histogram shows the relative activity of luciferase. t-test was used to assess statistically signifificant differences between groups.Mean ± SD, results of three independent experiments, *p < 0.05.