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. 2020 Feb 27;51:31. doi: 10.1186/s13567-020-00756-2

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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PCV2 infection in STEC. A Detection of PCV2 infection in STEC by indirect immunofluorescence assay. Green fluorescence indicates that cells were infected with PCV2. Scale bar, 100 μm. B Proliferation of PCV2 in STEC. The cells were infected with PCV2 at MOI 1, the supernatants and cells were frozen and thawed 3 times at 12 h, 24 h, 36 h and 48 h post-infection. Viral DNA were extracted and quantitated by qRT-PCR assay. The results are shown as mean ± SD of three separate experiments.