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. 2020 Feb 27;51:31. doi: 10.1186/s13567-020-00756-2

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Coinfection with PCV2 and SS2 causes down-regulation of TJ in STEC. STEC were infected with PCV2 for 24 h or 36 h before incubating with SS2 for 12 h. A Protein levels of ZO-1 and occludin were determined by Western blotting and band intensities relative to control were analyzed. B mRNA levels of ZO-1 and occludin were analyzed with qRT-PCR. The results were described as mean ± SD of three independent experiments. Significant differences were determined using one-way ANOVA analysis. *P < 0.05; **P < 0.01; ***P < 0.001; ns: not significant.