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. 2020 Feb 27;51:31. doi: 10.1186/s13567-020-00756-2

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Activation of JNK/MAPK pathway is related to the disruption of TJ and increased-permeability of tracheal epithelial barrier. A Western blotting analysis of p38, ERK and JNK phosphorylation in PCV2-infected STEC. STEC were infected with PCV2 for 36 h or 48 h. Phosphorylation of JNK was detected in the process of PCV2 infection. B Western blotting analysis of ZO-1 and occludin in PCV2-infected STEC treated with JNK inhibitor SP600125. Band intensities relative to control were analyzed. Compared with the PCV2-infected cells, the disruption of ZO-1 and occludin were alleviated through inhibition of JNK activation. CT, the control group. P, the PCV2 infection group. C Measurement of paracellular permeability. PCV2 infection increased the permeability of the epithelial barrier, which can be partially offset by JNK inhibitor. The results are described as mean ± SD of three independent experiments. Significant differences in (B) and (C) were determined using one-way ANOVA analysis. *P < 0.05; **P < 0.01; ***P < 0.001; ns, not significant. D Immunofluorescence staining of ZO-1 and occludin in STEC. Scale bar, 50 μm. The infection time of PCV2 was 48 h.