Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2020 Feb 21;10:170. doi: 10.3389/fonc.2020.00170

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2020 Jin, Liu, Liu, Li, Xu, He, Liu, Zhang and Ke.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

PMC Copyright notice

Identification of hsa_circ_0088732. (A) Fluorescence in situ hybridization (FISH) was performed to show the levels and locations of Lcn2-derived circRNA (hsa_circ_0088732) expression. Red indicates circRNA and blue indicates nuclei (DAPI). The white arrow indicates hsa_circ_0088732. Magnification, ×200; Scale bar = 100 μm. (B) The linear and circular Lcn2 in cDNA and gDNA were amplified by using convergent and divergent primers, respectively. (C) qRT-PCR was used to analyze the expression of GAPDH and hsa_circ_0088732 RNA after treatment with RNaseR. (D) Sanger sequencing was used to verify the circular structure of hsa_circ_0088732; red arrow shows the site of the backsplice junction.