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. 2020 Feb 21;10:170. doi: 10.3389/fonc.2020.00170

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Copyright © 2020 Jin, Liu, Liu, Li, Xu, He, Liu, Zhang and Ke.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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RAB3D served as a target of miR-661. (A) RAB3D mRNA expression in samples of normal and tumor tissues was examined by qRT-PCR. (B) Person's correlation coefficient was used to analyze the correlation between RAB3D and miR-661. (C,D) RAB3D expression was measured by qRT-PCR and western blot assays after transfection with miR-661 mimics (^**P < 0.01). (E) The binding site between RAB3D and miR-661 was identified. (F) 293T cells were co-transfected with WT-RAB3D or MUT-RAB3D and miR-661 or NC; dual luciferase reporter assays were used to analyze relative luciferase activity. (G) GEPIA showing the overall survival of glioma patients. (H) RAB3D expression in glioma and adjacent non-tumor tissues was assessed by immunochemistry (online database: The human protein atlas).