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. 2020 Feb 21;10:170. doi: 10.3389/fonc.2020.00170

Table 1.

The sequences of primers used in real-time PCR.

Gene Sequence(5′ - 3′)
GAPDH F TGTTCGTCATGGGTGTGAAC
GAPDH R ATGGCATGGACTGTGGTCAT
hsa_circ_0088732 F ATAAACATGTGCCCTCAGGC
hsa_circ_0088732 R TTGGGACAGGGAAGACGATG
U6 F CTCGCTTCGGCAGCACA
U6 R AACGCTTCACGAATTTGCGT
All R CTCAACTGGTGTCGTGGA
Hsa-miR-661 TGCCTGGGTCTCTGGCCTGCGCGT
Hsa-miR-661 RT CTCAACTGGTGTCGTGGAGTCGGCAATTCAGTTGAGACGCGCA
Hsa-miR-661 F ACACTCCAGCTGGGTGCCTGGGTCTCTGGCCTGC
Hsa-miR-7 TGGAAGACTAGTGATTTTGTTGTT
Hsa-miR-7 RT CTCAACTGGTGTCGTGGAGTCGGCAATTCAGTTGAGAACAACA
Hsa-miR-7 F ACACTCCAGCTGGGTGGAAGACTAGTGATTTTGT

F, forward primer; R, reversed primer; RT, reverse transcription primer.